New York, USA – July 28, 2022 – Profacgen, a world leader in serving life technology, recently enhanced its stable cell line construction service for protein expression, aiming to help scientists meet their biological development needs.
Stable cell lines are frequently employed in many crucial applications, such as the generation of biologics (like recombinant proteins and monoclonal antibodies), drug screening, and gene functional investigations.
For reliable and affordable protein production when a big quantity of recombinant protein is needed, stable expression line constructure is advised. A stable cell line is typically created in three steps: target gene delivery into the host cell, stable expression clone selection, and stability testing.
To assist clients in meeting their demands for biologics development, Profacgen offers development services that make use of cutting-edge technology and established knowledge. The company’s cell line development team works closely with the bioanalytical testing and manufacturing teams to provide high-performance, highly stable production cell lines in a variety of host cells within the quickest development cycle and to guarantee that processes and products are scalable to clinical and commercial scale.
For effective gene delivery and a high integration rate, Profacgen uses lentiviral vectors. Then, to choose for monoclones with stable expression and high yield, limited dilution and antibiotic screening are used. Profacgen can also employ flow cytometry to look for positive clones when fluorescent protein is fused to the target protein. In order to induce the expression of target gene, Profacgen additionally provides the creation of Tet-ON inducible stable cell lines.
To ensure integration of target gene and stable/inducible expression of the protein, Profacgen also provides PCR, qRT-PCR, microscopy, flow cytometry analysis and stability test based on diversified needs.
To know more detailed information about the updated stable cell line construction service for protein expression, please visit https://www.profacgen.com/stable-cell-line-construction.htm.